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  • Title: Production of flavonoid o-glucoside using sucrose synthase and flavonoid o-glucosyltransferase fusion protein.
    Author: Son MH, Kim BG, Kim DH, Jin M, Kim K, Ahn JH.
    Journal: J Microbiol Biotechnol; 2009 Jul; 19(7):709-12. PubMed ID: 19652519.
    Abstract:
    Enzymatic glucosylation with glycosyltransferases can be used to regulate the water solubility of aglycone. The drawback of this process is the demand of UDP-glucose as a sugar donor. We made an in-frame fusion of the flavonoid O-glucosyltransferase (OsUGT-3) and sucrose synthase (AtSUS) genes. The resulting fusion protein, OsUGT3-AtSUS, was expressed in E. coli and purified. When sucrose and UDP were supplied, SUS-UGT was able to convert quercetin into quercetin O-glucoside without the addition of UDP-glucose. In addition, UDPglucose was recycled when sucrose was added to the reaction mixture. This fusion protein is useful for the enzymatic production of flavonoid O-glucosides.
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