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  • Title: The hybridization of EBV-immortalized human B-lymphocytes with a human-mouse heteromyeloma cell line.
    Author: Jahn S, Walper A, Grunow R, Heym S, Volk HD, von Baehr R.
    Journal: Allerg Immunol (Leipz); 1990; 36(4):359-65. PubMed ID: 1965878.
    Abstract:
    The combination of Epstein-Barr-Virus (EBV)-permitted immortalization and somatic hybridization (fusion with a myeloma partner) may be the method of choice to produce human monoclonal antibodies. We show here that the fusion of EBV-infected human B-lymphocytes to the HAT-sensitive, ouabain-resistent heteromyeloma (human x mouse) fusion line CB-F7, resulted in stable growing hybridomas producing much more immunoglobulin than the parental lymphoblastoid lines. A more efficient clonability was shown for hybridoma cultures too. The loss of B cell markers (HLA-class II antigen, CD-22, CD-37) was detected. Limiting dilution experiments showed a better fusionability of IgM-producing EBV-transformed B cells in comparison to IgG-secreting counterparts.
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