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  • Title: Phosphatidylinositol-attached 5'-nucleotidase from synaptic plasma membrane of rat brain.
    Author: Lai KM, Wong PC.
    Journal: Biochem Int; 1990 Dec; 22(5):867-76. PubMed ID: 1965942.
    Abstract:
    Synaptic plasma membranes (SPM) of rat brain contained a 5'-nucleotidase that was specifically released by Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC). About 30% of the enzyme was readily released and the remainder was less susceptible. Purified 5'-nucleotidase was treated with PIPLC and the resultant enzyme was almost totally partitioned into the detergent-poor phase following phase-separation in Triton X-114 indicating that PIPLC converted the enzyme from an amphipathic to a hydrophilic form. The results suggest that 5'-nucleotidase is anchored into SPM by a covalently attached phosphatidylinositol moiety.
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