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  • Title: Optical detection of specific genes for genetically modified soybean and maize using multiplex PCR coupled with primer extension on a plastic plate.
    Author: Harikai N, Saito S, Abe M, Kondo K, Kitta K, Akiyama H, Teshima R, Kinoshita K.
    Journal: Biosci Biotechnol Biochem; 2009 Aug; 73(8):1886-9. PubMed ID: 19661710.
    Abstract:
    A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension primer-immobilized plate and the spots corresponding to the DNA sequences were visualized. This method is a rapid and simple way to detect GM soybean and GM maize optically.
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