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  • Title: Immobilization of penicillin G acylase onto ion exchange and hydrophobic resins.
    Author: Kumar KK, Ambekar GR, Shewale JG.
    Journal: Hindustan Antibiot Bull; 1990; 32(3-4):84-7. PubMed ID: 1966686.
    Abstract:
    Penicillin G acylase was immobilized on numerous cation exchange resins and hydrophobic adsorbents. Amberlite XAD-7 was the matrix of choice among the matrices studied for the immobilization of enzyme. Binding of 96.8% and expression of 82.6% of the penicillin G acylase was achieved on XAD-7. Penicillin G acylase immobilised on XAD-7 was used for 80 cycles for the production of 6-PA in a stirred tank reactor.
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