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  • Title: Identification of two somatostatin-immunoreactive cell types in the principal islet of Sparus auratus L. (Teleostei) by immunogold staining.
    Author: Abad ME, Lozano MT, Taverne-Thiele JJ, Rombout JH.
    Journal: Gen Comp Endocrinol; 1990 Jan; 77(1):1-8. PubMed ID: 1967240.
    Abstract:
    Two types of somatostatin (SST 14)-immunoreactive cells are identified by immunogold staining in the Lowicryl-embedded principal islet of Sparus auratus: D1 cells, having large moderate to low electron dense granules, located between A cells in the islet periphery and D2 cells, containing smaller electron-dense granules, present between B cells in the central region of the islet. Although SST 28-like immunoreactivity was not observed in D cells of S. auratus, the presence of SST 14 and a SST 22-,25-, or 28-like sequence in D2 and D1 cells, respectively, is discussed. A third SST 14-immunoreactive cell, found in the islet periphery, showed immunoreactive D1- and unreactive A-like granules. This cell type, which has a pyknotic-like nucleus and a dark appearance in osmicated Epon-embedded tissue, is supposed to be the product of fusion of D1 and A cells.
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