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Title: Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples. Author: Veron V, Simon S, Blanchet D, Aznar C. Journal: Diagn Microbiol Infect Dis; 2009 Sep; 65(1):69-72. PubMed ID: 19679239. Abstract: We report a TaqMan real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.[Abstract] [Full Text] [Related] [New Search]