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  • Title: Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis.
    Author: Rowley AH, Whitley RJ, Lakeman FD, Wolinsky SM.
    Journal: Lancet; 1990 Feb 24; 335(8687):440-1. PubMed ID: 1968171.
    Abstract:
    Herpes-simplex-virus (HSV) DNA in cerebrospinal fluid was amplified by use of the polymerase chain reaction and identified by hybridisation to a specific oligonucleotide probe. Specimens of cerebrospinal fluid (CSF) from 4 of 4 patients with herpes simplex encephalitis were positive for HSV DNA, whereas CSF specimens from 6 patients with other central-nervous-system infections were negative. This technique may expedite diagnosis of herpes simplex encephalitis.
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