These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: [Simultaneous detection of aflatoxin B1 and ochratoxin A by the dual-label time-resolved fluoroimmunoassay].
    Author: Huang B, Zhang J, Ma Z, Zhang Y.
    Journal: Wei Sheng Yan Jiu; 2009 Jul; 38(4):385-8. PubMed ID: 19689061.
    Abstract:
    OBJECTIVE: Using dual-label time-resolved fluoroimmunoassay (TRFIA) technology to establish a high sensitive method for simultaneously detect aflatoxin B1 (AFB1) and Ochratoxin A (OTA). METHODS: AFB1-HRP and OTA-BSA coated 96-well plates were taken as the solid phase antigen to compete with free AFB1 and OTA for limited anti-AFB1 polyclonal antibody or anti-OTA monoclonal antibody. Then rare earth ion Eu3+ labeled goat anti-rabbit antibody and Sm3+ labeled goat anti-mouse antibody were applied as tracers to establish AFB1/ OTA-TRFIA in dissociation enhanced fluorescence immunoassay system by using indirect competitive immunoassay. RESULTS: For this method, the sensitivity of AFB1 was 0.02 microg/L with a measurement range of 0.02-100 microg/L, intra-and inter-assay CV of 3.2% and 7.3%, and an average recovery rates of 88.1%. While the sensitivity of OTA was 0.051 microg/L with a measurement range of 0.05-50 microg/L, intra-and inter-assay CV of 2.9% and 7.9%, and an average recovery rates of 89.9%. In AFB1/ OTA-TRFIA detection, the AFB1 and OTA did not interfere with each other. When samples were detected by dual-label AFB1/ OTA-TRFIA, single label AFB1-TRFIA and OTA-TRFIA at the same time, the AFB1/ OTA-TRFIA detection and AFB1-TRFIA detection for AFB1 were correlated by 0.972, while the correlation coefficient of AFB1 /OTA-TRFIA detection and OTA-TRFIA detection for OTA was 0.981, indicating that the results were consistent. CONCLUSION: The study showed that AFB1/ OTA-TRFIA, with high sensitivity, wide measuring range, good stability, and simultaneous detection of AFB1 and OTA, could be a simple, fast and economic method.
    [Abstract] [Full Text] [Related] [New Search]