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  • Title: Amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation for hydrolysis of Cassava starch.
    Author: Chen L, Chi ZM, Chi Z, Li M.
    Journal: Appl Biochem Biotechnol; 2010 Sep; 162(1):252-63. PubMed ID: 19701612.
    Abstract:
    The optimization of process parameters for high amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation was carried out using central composite design. Finally, the optimal parameters obtained with the response surface methodology (RSM) were moisture 610.0 ml/kg, inoculum 30.0 ml (OD(600 nm) = 20.0)/kg, the amount ratio of wheat bran to rice husk 0.42, cassava starch concentration 20.0 g/kg, temperature 28 degrees C, and natural pH. Under the optimized conditions, 4,296 U/g of dry substrate of amylase activity was reached in the solid-state fermentation culture of the yeast strain A11 within 160 h, whereas the predicted maximum amylase activity of 4,222 U/g of dry substrate of amylase activity was derived from the RSM regression. It was found that cassava starch can be actively converted into monosaccharides and oligosaccharides by the crude amylase.
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