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  • Title: ERIC-PCR-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems.
    Author: Wang L, Jin Y, Zhao L, Pang X, Zhang X.
    Journal: Lett Appl Microbiol; 2009 Oct; 49(4):522-8. PubMed ID: 19708883.
    Abstract:
    AIMS: To evaluate the use of Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR)-derived probes and primers to specifically detect bacterial strains in an activated sludge microbial community. METHODS AND RESULTS: ERIC-PCR was performed on two phenol-degrading bacterial strains, Arthrobacter nicotianae P1-7 and Klebsiella sp. P8-14. Their amplicons were DIG labelled for use as probes and then hybridized with ERIC-PCR fingerprints. The results showed the distinct band patterns for both bacterial strains. Strain-specific PCR primers were designed based on the sequences of ERIC-PCR bands. The DNA of each of these strains was successfully detected from its mixture with activated sludge DNA, either by using their respective ERIC-PCR-based probes for hybridization or by using species-specific primers for amplification, with higher sensitivity by latter method. CONCLUSIONS: Two phenol-degrading bacterial strains were identified from a mixture of activated sludge by using ERIC-PCR-based methods. SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrated that the bacteria, which have important functions in complex wastewater treatment microbial communities, could be specifically detected by using ERIC-PCR fingerprint-based hybridization or amplification.
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