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  • Title: Detection of rotavirus in food associated with a gastroenteritis outbreak in a mother and child sanatorium.
    Author: Mayr C, Strohe G, Contzen M.
    Journal: Int J Food Microbiol; 2009 Oct 31; 135(2):179-82. PubMed ID: 19717201.
    Abstract:
    In the course of a rotavirus outbreak in a mother and child sanatorium 74 food samples from the sanatorium kitchen were taken and tested for rotavirus. Rotavirus particles were isolated from 25 g food samples by a simple method including ultrafiltration, originally designed for the detection of norovirus in various food matrices. Rotavirus was successfully detected in a sample of potato stew by conventional RT-seminested-PCR. Sequence comparison of the amplification products obtained from the potato stew and a stool sample from an infected child verified that the two viruses were identical.
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