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  • Title: AmBisome and Amphotericin B inhibit the initial adherence of Candida albicans to human epithelial cell lines, but do not cause yeast detachment.
    Author: Dorocka-Bobkowska B, Düzgüneş N, Konopka K.
    Journal: Med Sci Monit; 2009 Sep; 15(9):BR262-9. PubMed ID: 19721394.
    Abstract:
    BACKGROUND: Candida biofilms with reduced susceptibility to conventional antifungals are sensitive to lipid formulations of amphotericin B (AMB). We examined the effect of the liposomal AMB formulation, AmBisome, and free AMB on the adherence of C. albicans to HeLa cervical carcinoma and HSC-3 oral squamous cell carcinoma cells. MATERIAL/METHODS: HeLa and HSC-3 cells were incubated with three oral isolates of C. albicans either in the presence of AmBisome or AMB, or pre-incubated with yeasts and subsequently exposed to the drug. The effect of the drugs on the viability of HeLa and HSC-3 cells was determined by an Alamar Blue assay. RESULTS: Following a 1-h incubation in the presence of AmBisome, at 1-256 microg/ml, the adherence of C. albicans to HeLa and HSC-3 cells was reduced considerably. For example at 16 microg/ml, adherence was diminished by approximately 66% in HeLa and by approximately 36% in HSC-3 cells. The susceptibility of cell-associated Candida to antifungals was decreased markedly. The reduction in adherence was between 3.3 and 13.7%, when compared to the drug-free controls. AmBisome was not toxic in the range 1-256 microg/ml, while free AMB was not toxic at 1 and 4 microg/ml to HeLa cells and at 1, 4 and 16 microg/ml to HSC-3 cells. CONCLUSIONS: AmBisome inhibited candidal attachment when present during the "adherence phase" but did not cause detachment of cell-associated yeasts. The effect of AmBisome on candidal adherence to HSC-3 cells was less inhibitory than that observed with HeLa cells. Candidal adherence to epithelial cells is significantly reduced when antifungal polyenes are present during the "adherence phase", while cell-associated Candida is resistant to antifungals in terms of adherence.
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