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  • Title: HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.
    Author: Wittek R, Menna A, Schümperli D, Stoffel S, Müller HK, Wyler R.
    Journal: J Virol; 1977 Sep; 23(3):669-78. PubMed ID: 197263.
    Abstract:
    The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.
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