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  • Title: Altered acinar distribution of glutamine synthetase and different growth response of cultured enzyme-positive and -negative hepatocytes after partial hepatectomy.
    Author: Gebhardt R.
    Journal: Cancer Res; 1990 Jul 15; 50(14):4407-10. PubMed ID: 1973072.
    Abstract:
    Partial hepatectomy (PH) results in the persistent drop of the specific activity of glutamine synthetase (GS) (EC 6.3.1.2). This drop correlates with the reduced proportion of GS+ hepatocytes and with the reduced GS+ area surrounding the central veins such that GS+ hepatocytes are arranged in a single cell layer only. Cultivation of hepatocytes isolated at various times after PH revealed considerable differences in the growth characteristics of GS+ and GS- hepatocytes discriminated by immunocytochemistry. In the absence or presence of epidermal growth factor and insulin, the labeling index of GS- hepatocytes peaked in cultures established 48 h after PH at 10% and 50%, respectively, while that of GS+ cells was much lower (2% and 6%). In cultures established at later times after PH the labeling index of GS- cells decreased gradually, while that of GS+ hepatocytes increased continuously, reaching about 20% and more than 50% for controls and epidermal growth factor/insulin-treated cultures, respectively, in cultures established 72 after PH. Norepinephrine stimulated the labeling index of both cell populations during the first 24 h only, but again GS- hepatocytes responded somewhat earlier than did GS+ hepatocytes. These results demonstrate that the differences in the growth characteristics of GS+ and GS- hepatocytes are due to different priming of these cells in vivo and may result in the different expansion of the respective cell populations during regeneration after PH.
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