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  • Title: Development of a downscale sedimentation field flow fractionation device for biological event monitoring.
    Author: Bégaud-Grimaud G, Battu S, Liagre B, Beneytout JL, Jauberteau MO, Cardot PJ.
    Journal: J Chromatogr A; 2009 Dec 25; 1216(52):9125-33. PubMed ID: 19732901.
    Abstract:
    Classically described as a macroscale size-density based method, Sedimentation field flow fractionation (SdFFF) has been successfully used for cell sorting. The goal of this study was to develop a new SdFFF device for downscale applications, in particular for oncology research to rapidly monitor chemical biological event induction in a cell line. The development of a downscale SdFFF device required reduction of the separation channel volume. Taking advantage of a newly laboratory designed apparatus, channel volume was successfully decreased by reducing both length and breadth. To validate the apparatus and method, we used the well-known model of diosgenin dose-dependent induction of apoptosis or megakaryocytic differentiation in HEL cells. After a minute scale acquisition of a reference profile, the downscale device was able to perform fast, early, significant and reproducible monitoring of apoptosis and differentiation, two important biological mechanisms in the field of cancer research.
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