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  • Title: Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides.
    Author: Qu Y, Sun Q, Xiao F, Shi G, Jin L.
    Journal: Bioelectrochemistry; 2010 Feb; 77(2):139-44. PubMed ID: 19733130.
    Abstract:
    In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8x10(-9)M to 0.9x10(-7)M was exhibited with a detection limit of 4.0x10(-9)M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.
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