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  • Title: Biomimetic triblock copolymer membrane arrays: a stable template for functional membrane proteins.
    Author: González-Pérez A, Stibius KB, Vissing T, Nielsen CH, Mouritsen OG.
    Journal: Langmuir; 2009 Sep 15; 25(18):10447-50. PubMed ID: 19735126.
    Abstract:
    It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 microm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.
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