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  • Title: Effect of calcium-modifying drugs on mouse in vitro fertilization and preimplantation development.
    Author: Blancato JK, Seyler DE.
    Journal: Int J Fertil; 1990; 35(3):171-6. PubMed ID: 1973923.
    Abstract:
    Calcium ions are required for normal in vitro fertilization and preimplantation development in the mouse. This study examined the effects of alterations in Ca2+ flux and distribution on sperm penetration of eggs and embryo cleavage. Compounds used included diltiazem, a Ca2+ channel blocker, and 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), an antagonist of intracellular calcium release. Incubation of sperm and eggs with diltiazem at 30 and 10 microM and TMB-8 at 30, 10, and 3 microM resulted in depressed fertilization compared with controls. Motility was not inhibited at these concentrations of either drug. Both drugs also depressed progression of mouse embryos from 2-cell to blastocyst at 30 and 3-microM concentrations. This study suggests that both Ca2+ flux and distribution to specific cellular sites are required for normal mouse in vitro fertilization and early preimplantation development.
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