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  • Title: A novel method to detect seven microcystins in hard clam and corbicula fluminea by liquid chromatography-tandem mass spectrometry.
    Author: Yang B, Xu JZ, Ding T, Wu B, Jing S, Ding SJ, Chen HL, Sheng CY, Jiang Y.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 Nov 01; 877(29):3522-8. PubMed ID: 19748327.
    Abstract:
    A simple and reliable method to detect seven microcystins in hard clam and corbicula fluminea, based on liquid chromatography with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS), was developed and validated. The sample preparation procedure includes extraction of tissue by methanol, followed by cleanup on a reversed-phase solid phase extraction (SPE) cartridge. With the optimized method, recoveries were between 43.7% and 92.3% for hard clam, 54.3% and 93.8% for corbicula fluminea, the relative standard deviations (RSD) were less than or equal to 16.2% and 15.7% in hard clam and corbicula fluminea at spiking levels of 1 microg/kg, 2 microg/kg and 5 microg/kg for MC-RR, MC-YR, MC-LR, and MC-LY, and 2 microg/kg, 5 microg/kg and 10 microg/kg for MC-LA, MC-LW and MC-LF, respectively, the limits of quantitation (LOQ) of this method were ranged from 0.7 microg/kg to 2.0 microg/kg.
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