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  • Title: Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase.
    Author: Niwa K, Nakajima Y, Ohmiya Y.
    Journal: Anal Biochem; 2010 Jan 15; 396(2):316-8. PubMed ID: 19748476.
    Abstract:
    Based on the biosynthetic pathway of firefly bioluminescence substrate D-luciferin, the concentration of L-cysteine can be quantified using a simple protocol and a conventional luminescence detector. The lower limit of quantification (signal/noise ratio [S/N]=10) was 0.26 microM. Using our method, the total amount of free/reduced and disulfide/oxidized L-cysteine could be measured successfully in human serum. In addition, biosynthetic precursors such as 2-cyano-6-hydroxybenzothiazole and L-luciferin could replace d-luciferin in the cell-based luciferase assay. Our results suggest that the bioluminescence reaction associated with the biosynthesis of bioluminescence substrates can provide a fast and cost-effective assay method.
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