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  • Title: Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis.
    Author: Picardeau M.
    Journal: Genetica; 2010 May; 138(5):551-8. PubMed ID: 19757097.
    Abstract:
    Mariner eukaryotic elements transpose randomly and independently of any host factors, making them ideal tools for random mutagenesis in bacteria, including genetically intractable microorganisms. The transposable element Himar1, a member of the mariner family of transposons, originally isolated from the horn fly (Haematobia irritans), has thus been extensively used to generate large numbers of insertion mutants. Transposon-based approaches greatly facilitate studies of bacterial biology. We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria.
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