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  • Title: [HPLC fingerprint of flavonoids and phenols of Dendrobium nobile].
    Author: Ou HJ, Cheng JL, Li XX, Zhan RT, Liang YZ, Xu JY, Xu H, Yan P.
    Journal: Zhong Yao Cai; 2009 Jun; 32(6):871-4. PubMed ID: 19764325.
    Abstract:
    OBJECTIVE: To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile. METHODS: Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm. RESULTS: The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed. CONCLUSION: This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.
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