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  • Title: Cryopreservation of in vitro shoot apices of Glehnia littoralis - a medical plant.
    Author: Otokita K, Kami D, Suzuki T, Suzuki M.
    Journal: Cryo Letters; 2009; 30(4):244-50. PubMed ID: 19789821.
    Abstract:
    Cryopreservation was examined as a practical method for preserving the genetic resources of Glehnia littoralis Fr. Schm. a nearly exterminated medical plant. In vitro shoot apices were successfully cryopreserved using vitrification and encapsulation-dehydration. In vitrification, regrowth of apices loaded for 60 min with PVS2 (66.7 +/- 6.7 percent) was preferable to loading with PVS3 (50.0 +/- 5.8 percent). With encapsulation-dehydration, the best regrowth (43.3 +/- 3.3 percent) was achieved when the moisture content in the beads was reduced to 19 percent by drying with silica gel for 6 h. Increased regrowth of shoot tips cryopreserved by encapsulation-dehydration resulted from the addition of 0.1 mM acetylsalicylic acid to the loading solution (86.7 +/- 3.3 percent).
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