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  • Title: Modulation of enzyme-substrate selectivity using tetraethylene glycol functionalized gold nanoparticles.
    Author: Jordan BJ, Hong R, Han G, Rana S, Rotello VM.
    Journal: Nanotechnology; 2009 Oct 28; 20(43):434004. PubMed ID: 19801753.
    Abstract:
    Tetraethylene glycol (TEG) functionalized gold nanoparticles with 2 nm core diameters (AuTEG) enhance alpha-chymotrypsin (ChT) enzyme activity in a substrate-selective fashion. We explored the hydrolysis of four different substrates and observed a marked increase in activity with the most hydrophobic substrate N-succinyl-alanine-alanine-proline-phenylalanine- p-nitroanilide (TP), while the other substrates remain virtually unaffected by the AuTEG 'crowding effect' in solution. The enhancement in catalysis is indicated by an increase in K(cat)/K(m) as obtained from Lineweaver-Burk analysis and we hypothesize it to arise from a macromolecular crowding effect analogous to that observed with high molecular weight poly(ethylene glycol) (PEG) polymers.
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