These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: MCE enzyme immunoassay for carcinoembryonic antigen and alpha-fetoprotein using electrochemical detection.
    Author: Zhang S, Cao W, Li J, Su M.
    Journal: Electrophoresis; 2009 Oct; 30(19):3427-35. PubMed ID: 19802853.
    Abstract:
    An MCE electrochemical enzyme immunoassay protocol for the determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was reported. Two antigens (Ag), CEA and AFP, were incubated simultaneously with an excess amount of horseradish peroxidase-labeled antibody (Ab(*)). The free Ab(*) and the Ab(*)-Ag complex produced in the solution were first separated through a postcolumn reaction and then traced by the enzyme substrate o-aminophenol. The 3-aminophenoxazine produced in enzyme reaction was detected with downstream amperometric detection. The separations were performed at a separation voltage of +1.4 kV and were completed in less than 60 s. The better analytical performance and distinct miniaturization/portability for MCE at less assay time and sample volume consumption was achieved. The detection limit of CEA and AFP was calculated to be 0.25 and 0.13 ng/mL, respectively. Therefore, MCE could be used as a sensitive and new tool in separation science and offered considerable promise in biological sample analysis or quick clinical diagnosis.
    [Abstract] [Full Text] [Related] [New Search]