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  • Title: Culture of mature hippocampus slices for 4 days in a newly developed medium: preservation of transmitter release and leucine incorporation into protein.
    Author: Kleinberger-Doron N, Schramm M.
    Journal: Brain Res; 1990 Nov 19; 533(2):239-47. PubMed ID: 1981168.
    Abstract:
    A procedure and a medium for sustaining mature hippocampus slices in vitro for 4 days are described. The ionic composition of the medium, in which the slices were incubated for 1 h of recovery following preparation, strongly affected their ability, 4 days later, to take up and to release D-[3H]aspartate and [14C]GABA. A medium deficient in Na+ and Ca2+ proved best for recovery of the fresh slices prior to transfer to culture medium. The newly developed CSF-like culture medium was the best among several media tested in maintaining the potential of the slices for uptake and for induced release of D-[3H]aspartate and [14C]GABA. Glutamine, present in most culture media, appeared to be particularly toxic. Relative to fresh slices, the slices after 4 days in culture maintained 118% and 97% of the uptake of D-[3H]aspartate and of [14C]GABA respectively. K(+)-induced release of D-[3H]aspartate and of [14C]GABA was 104% and 82% of the respective values in fresh slices. Under the optimal culture conditions worked out, the slices also regained a considerable capacity for incorporation of labelled leucine into protein, which was low in fresh slices.
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