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  • Title: [Activation of transcription of the tyrosine aminotransferase gene in the rat McA-RN 7777 hepatoma cell line by cycloheximide].
    Author: Kiseleva NP, Polotskaia AV, Adler VV.
    Journal: Mol Biol (Mosk); 1990; 24(5):1255-60. PubMed ID: 1981248.
    Abstract:
    The expression of the tyrosine aminotransferase (TAT) mRNA after cycloheximide treatment was analysed by Northern blotting method in Morris rat hepatoma cell lines. The level of TAT mRNA increased after 6-8 h of cycloheximide treatment only in the McA-RH 7777 cell line. McA-RH 7777 nuclear run-off assay showed that TAT transcription was induced by cycloheximide treatment. Both glucocorticoid and cycloheximide modulated TAT gene transcription in a synergistic way. There was no induction of TAT expression following cycloheximide or cycloheximide glucocorticoid simultaneous treatment in another cell line (McA-RH 8994), while c-myc and c-fos expression was superinduced by cycloheximide treatment. The possible mechanism of transcription regulation and its damage in hepatoma cells is discussed.
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