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  • Title: Detection and evaluation of feline oncornavirus-induced cell surface antigen(s) shed from cells in vitro.
    Author: Wolff LH, Mathes LE, Schaller JP, Hoover EA, Olsen RG.
    Journal: Cancer Res; 1977 Nov; 37(11):4134-7. PubMed ID: 198129.
    Abstract:
    A method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. This technique relied upon the natural release of antigen(s) from FL-74 feline lymphoblastoid cells during their maintenance at 37 degrees in serum-deficient medium. When concentrated and clarified spent medium from 4-day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, it was found that this natural production of soluble antigen provided more feline oncornavirus-associated cell membrane antigen per cell than did a solubilization procedure in which papain was used. The shed antigen preparation was immunogenic in cats, eliciting humoral antibody that was reactive with the surface of FL-74 Cells and feline sarcoma virus-transformed nonproducer mink cells but was not reactive with feline leukemia virus in a virus neutralization assay.
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