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  • Title: Binding and elution behavior of proteins on strong cation exchangers.
    Author: Pabst TM, Suda EJ, Thomas KE, Mensah P, Ramasubramanyan N, Gustafson ME, Hunter AK.
    Journal: J Chromatogr A; 2009 Nov 06; 1216(45):7950-6. PubMed ID: 19815222.
    Abstract:
    This work provides a broad survey of binding and elution behavior of proteins on strong cation exchangers. Four proteins comprising two monoclonal antibodies, lysozyme, and cytochrome c were used as models in the investigation. Seven chromatography resins with different base matrices were compared. Dynamic binding capacity as a function of salt concentration was examined for a monoclonal antibody and lysozyme. Elution behavior as a function of gradient slope was modeled to determine the characteristic charge, essentially a measure of the number of sites involved in binding, for each protein on each resin. Trends with respect to dynamic binding capacity and elution behavior are analyzed and discussed.
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