These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Development and validation of a liquid chromatography-tandem mass spectrometry assay for serum 25-hydroxyvitamin D2/D3 using a turbulent flow online extraction technology.
    Author: Bunch DR, Miller AY, Wang S.
    Journal: Clin Chem Lab Med; 2009; 47(12):1565-72. PubMed ID: 19842995.
    Abstract:
    BACKGROUND: Vitamin D is important to health and disease. Liquid chromatography-tandem mass spectrometry (LC-MSMS) is considered the most accurate technology for quantification of serum 25-hydroxyvitamin D (25OHD) which is the best biomarker for estimating vitamin D nutritional status. METHODS: Serum was mixed with acetonitrile containing hexadeuterated 25-hydroxyvitamin D(3) (d(6)-25OHD3) and centrifuged 10 min at 15,634Xg. The supernatant was injected onto a turbulent flow preparatory column then transferred to a polar endcapped C18 analytical column. The mass spectrometer was set for positive atmospheric pressure chemical ionization. RESULTS: The analytical cycle time was 5.5 min. Inter- and intra-assay CV for both analytes across three concentrations ranged from 3.8% to 14.2%. The method was linear from 3.0 to 283.6 nmol/L for 25-hydroxyvitamin D(3) (25OHD3) and 4.6 to 277.9 nmol/L for 25-hydroxyvitamin D(2) (25OHD2), with an accuracy of 88.7%-118.7% and 90.7%-100.3%, respectively. No carryover or ion suppression was observed. Comparison with a radioimmunoassay using patient specimens (n=527) showed a mean difference of 5.2%, and diagnostic agreement of 80.9% with Deming regression of slope 0.867, intercept 12.8, standard error of estimate (SEE) 17.4, and r=0.8425. CONCLUSIONS: The LC-MSMS method coupled with turbulent flow technology for serum 25OHD quantitation is rapid, efficient, and suitable for clinical testing.
    [Abstract] [Full Text] [Related] [New Search]