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  • Title: Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier.
    Author: Zhang LY, Sun MX.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 Dec 01; 877(31):4051-4. PubMed ID: 19858002.
    Abstract:
    A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM NDA, 20 mM borate buffer (pH 9.2) with the reaction time of 4 min at room temperature. The capillary electrophoresis analysis of GSH could be achieved in less than 120 s using 10 mM sodium tetraborate (pH 9.2) containing 2.5 mM beta-cyclodextrin (beta-CD) as the running buffer, and the detection limit of 2.5 x 10(-9) M (S/N=3) was obtained. This method was successfully applied to analyze the content of GSH in tobacco BY-2 cells.
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