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  • Title: Sister-chromatid exchange analysis in vivo using different 5-bromo-2'-deoxyuridine-labeling systems.
    Author: Madrigal-Bujaidar E, Sánchez-Sánchez MA.
    Journal: Mutat Res; 1991 Jan; 262(1):15-9. PubMed ID: 1986279.
    Abstract:
    The quality of sister-chromatid differentiation, the basal rate of sister-chromatid exchanges (SCE) and the rate of cellular proliferation were studied in untreated and mitomycin C(MMC)-treated mice, using 4 different systems for administering 5-bromodeoxyuridine (BrdU): BrdU adsorbed to charcoal, tablets of BrdU mixed with cholesterol, tablets of BrdU coated with agar and tablets of BrdU partially coated with paraffin. The quality of sister-chromatid differentiation with the studied methods showed a useful stain contrast in an average of 75.4% second-division mitosas, with the lowest average occurring in mice implanted with agar-coated tablets. The frequency of SCE and the replicative index were similar in mice administered BrdU by all 4 systems both in control and in MMC-treated mice. From a practical point of view, the charcoal method could be done the fastest.
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