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  • Title: Biolabeling with 2,4-dichlorophenoxyacetic acid derivatives: the 2,4-D tag.
    Author: Bade S, Röckendorf N, Franek M, Gorris HH, Lindner B, Olivier V, Schaper KJ, Frey A.
    Journal: Anal Chem; 2009 Dec 01; 81(23):9695-702. PubMed ID: 19873999.
    Abstract:
    Many bioanalytic and diagnostic procedures rely on labels with which the molecule of interest can be tracked in or discriminated from accompanying like substances. Herein, we describe a new labeling and detection system based on derivatives of 2,4-dichlorophenoxyacetic acid (2,4-D) and anti-2,4-D antibodies. The 2,4-D system is highly sensitive with a K(D) of 7 x 10(-11) M for the hapten-antibody pair, can be used on a large variety of biomolecules such as proteins, peptides, carbohydrates, and nucleic acids, is not hampered by endogenous backgrounds because 2,4-D is a xenobiotic, and is robust because 2,4-D is a very stable compound that withstands the conditions of most reactions usually performed on biomolecules. With this unique blend of properties, the 2,4-D system compares favorably with its rivals digoxigenin (DIG)/anti-DIG and biotin/(strept)avidin and provides an interesting and powerful tool in biomolecular labeling.
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