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  • Title: The relationship between transforming growth factor beta1 expression and cold ischemia injury of rat donor kidney.
    Author: He W, Liu K, Ding H.
    Journal: Exp Mol Pathol; 2010 Feb; 88(1):206-9. PubMed ID: 19874817.
    Abstract:
    OBJECTIVE: Cold ischemia injury represents an independent risk factor which favors chronic allograft nephropathy (CAN). In order to investigate the role of transforming growth factor-beta 1( (TGF-beta1) in the progression of CAN, we studied the relationship between the expression of TGF-beta1 and cold ischemia injury in the renal tubular epithelia of rat donor kidney. METHODS: A total of 24 Wistar rats were used in this study. In terms of the time of cryopreservation of donor kidney, the 24 Wistar rats were randomly divided into 3 groups: 0 h group(control group), 24 h group, 48 h group. A block removal of donor kidney with in situ perfusion of cooling HC-A preservation solution was adopted. The rat kidney was preserved 0 h, 24h and 48 h at 0-4 degrees C respectively. The morphologic changes of proximal tubular epithelial cells in different cryopreservation time were observed under light microscope and transmission electron microscope. The expression of TGF-beta1 mRNA and protein in proximal tubular epithelial cells of different cryopreservation time group were detected by in situ hybridization and immunohistochemistry analysis. RESULTS: 1. In 24 h group, part of the proximal tubular epithelial cells showed slight degeneration. In 48 h group, the proximal tubular epithelial cells demonstrated severe hydropic degeneration and part of the cells developed necrosis and effluxion. 2. Only a small amount of TGF-beta1 protein and mRNA were expressed in the renal tubular epithelial cells of 0 h group. The positive unit (PU) value of TGF-beta1 protein and mRNA were 6.37+/-2.77 and 5.29+/-2.15, respectively. As the cold ischemia time prolonged, the PU value of TGF-beta1 protein increased at 24 h group (10.20+/-3.27) and 48 h group (17.17+/-3.96) . The PU value of TGF-beta1 mRNA also increased at 24 h group (11.31+/-3.34) and 48 h group (19.01+/-3.53). There was the significant difference of TGF-beta1 protein PU value or mRNA PU value among these groups(P<0.05). CONCLUSION: There was the significant correlation between the expression of TGF-beta1 and the degree of cold ischemia injury. The results suggest that TGF-beta1 might play the key role in regeneration and reparation of renal tubular epithelial cell injury. The overexpression of TGF-beta1 might be one of the mechanisms that initiate chronic allograft nephropathy.
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