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  • Title: [Ru(bpy)2dppz]2+ electrochemiluminescence switch and its applications for DNA interaction study and label-free ATP aptasensor.
    Author: Hu L, Bian Z, Li H, Han S, Yuan Y, Gao L, Xu G.
    Journal: Anal Chem; 2009 Dec 01; 81(23):9807-11. PubMed ID: 19891459.
    Abstract:
    [Ru(bpy)2dppz]2+ electrochemiluminescence (ECL) was studied, and it was used to investigate DNA interaction and develop a label-free ATP aptasensor for the first time. ECL of [Ru(bpy)2dppz]2+ is negligible in aqueous solution, and increases approximately 1000 times when [Ru(bpy)2dppz]2+ intercalates into the nucleic acid structure. The ECL switch behavior of [Ru(bpy)2dppz]2+ is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. The ECL switch by DNA was applied to investigate the interaction of [Ru(bpy)2dppz]2+ with herring sperm DNA. The calculated equilibrium constant (K) is 1.35 x 10(6) M(-1), and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. Moreover, ATP can dramatically affect ECL of the [Ru(bpy)2dppz]2+/ATP aptamer complex. As a result, a label-free, sensitive, and selective [Ru(bpy)2dppz]2+ ECL method for ATP detection was developed. The detection limit is 100 nM for ATP (with a signal-to-noise ratio, S/N, of 3) with a linear range of 0-1 microM. The result demonstrates that [Ru(bpy)2dppz]2+ ECL holds great promise in aptasensors.
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