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  • Title: [An improved method to recover high quality genomic DNA from paraffin-embedded tissues].
    Author: Yilike X, Yang X, Wu G, Ma Q, Abudula A.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2009 Oct; 34(10):1042-8. PubMed ID: 19893259.
    Abstract:
    OBJECTIVE: To establish a high quality genomic DNA preparation method from formalin fixed and paraffin embedded tissues (FFPET) by integrating previous DNA extraction methods. METHODS: We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection. RESULTS: The improved genomic DNA extraction method combined the advantages of the water-bath de-waxing and DNA affinity column, making it possible to get high quality genomic DNA from paraffin embedded cervical cancer tissues, and especially efficient to recover genomic DNA fragments larger than 20 kb. CONCLUSION: The improved DNA extraction method is fast and convenient to recover high quality genomic DNA from paraffin embedded tissues.
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