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  • Title: Kaempferol glycosides with antioxidant activity from Brassica juncea.
    Author: Jung HA, Woo JJ, Jung MJ, Hwang GS, Choi JS.
    Journal: Arch Pharm Res; 2009 Oct; 32(10):1379-84. PubMed ID: 19898800.
    Abstract:
    From the leaves of Brassica juncea, three kaempferol glycosides, kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), kaempferol-3-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (2), and kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) were isolated and the structures elucidated on the basis of spectral and chemical evidences. Antioxidant activities were determined by measuring the scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO(-)). Compounds 1 and 3 showed good antioxidant activities with respective IC(50) values of 28.61 and 36.93 microM toward DPPH; respective IC(50) values of 9.79 and 11.40 microM toward ONOO(-). However, compound 2 showed no DPPH scavenging activity and weak ONOO(-) scavenging activity with an IC(50) value of 32.00 microM.
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