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  • Title: Detection of Staphylococcus lugdunensis by a new species-specific PCR based on the fbl gene.
    Author: Pereira EM, Oliveira FL, Schuenck RP, Zoletti GO, Dos Santos KR.
    Journal: FEMS Immunol Med Microbiol; 2010 Mar; 58(2):295-8. PubMed ID: 19912343.
    Abstract:
    Staphylococcus lugdunensis are unusually virulent coagulase-negative staphylococci associated with skin infections and endocarditis. We developed an accurate and simple PCR assay to identify S. lugdunensis isolates based on detection of the fbl gene, which encodes a fibrinogen-binding protein involved in pathogen adhesion. The PCR assay was established using 16 reference strains of different Staphylococcus species and further validated with a collection of 63 clinical staphylococcal isolates that were also phenotypically characterized. Reliable results for the detection of S. lugdunensis isolates were obtained for 100% of the strains evaluated, indicating that this PCR assay can be used in the routine of microbiology laboratories as one more tool for correct species differentiation.
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