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  • Title: [Simultaneous determination of tetracyclines, sulfonamides and quinolones residues in chicken livers by ultra performance liquid chromatography-tandem mass spectrometry].
    Author: Guo L, Zhu K, Jiang H, Li J, Li X, Ding S.
    Journal: Se Pu; 2009 Jul; 27(4):412-6. PubMed ID: 19938494.
    Abstract:
    An analytical method for the simultaneous determination of 3 tetracyclines, 10 sulfonamides and 8 quinolones in chicken livers by ultra performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UPLC-MS/MS) in positive ion mode with multiple reaction monitoring (MRM) has been developed and validated. A total of 2 g homogenized sample of chicken livers was placed in a 50 mL polypropylene tube, and 1 mL of McIlvaine buffer and 4 mL of acetonitrile were added. After stirring and centrifuging for 5 min at 4,000 r/min, the supernatant was collected and the remains was extracted by 5 mL acetonitrile. The supernatant was merged together and evaporated to dryness under a steam of nitrogen at 60 degrees C. The residue was dissolved with 4 mL of phosphoric acid-triethylamine buffer-acetonitrile (85:15, v/v) and 4 mL of n-hexane. After stirring for 1 min and centrifuging for 5 min at 4 000 r/min, the under layer solution was analyzed using UPLC-MS/MS. The satisfactory recoveries (66.8% - 128.5%) of all the veterinary drugs were demonstrated at spiked levels of 5, 10 and 50 microg/kg with the overall relative standard deviations (RSDs) for intra-day and inter-day of the 21 analytes less than 20.5%. The limit of detection (LOD) and the limit of quantification (LOQ) were 2 microg/kg and 5 microg/kg, respectively for each drug. This method has good stability, lower detection limits and can be used as a conclusive evidence method of these drug residues in chicken livers.
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