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  • Title: [Tissue culture and plant regeneration of Ardisia crenata].
    Author: Ma M, Liu J, Pu S.
    Journal: Zhongguo Zhong Yao Za Zhi; 2009 Aug; 34(16):2043-6. PubMed ID: 19938541.
    Abstract:
    OBJECTIVE: Our research studied the fast-breeding technology of Ardisia crenata sims by using tissue culture and provided the scientific foundation for industry production. METHOD: The effects of axillary buds and plant regeneration of different basic medium, hormones and additives on induction and multiplication were studied. RESULT: The best culture medium for the induction of axillary buds, which took the stems of A. crenate were as explants, was MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1), and the best medium for multiplication was MS + 6-BA 2.0 mg x L(-1) + NAA 0.1 mg x L(-1) + KT 0.5 mg x L(-1), the best medium for roots generation was 1/2MS + IBA 0.2 mg x L(-1). We also found that the roots'generation, roots rate and mean number of roots can be promoted by adding 0.2% Ac, and the most suitable ground substance was river sand-perlite-vermiculite (1:1:1) or perlite-vermiculite (1:1). With axillary buds and plant regeneration methode, more than 80% A. crenata sims could be regenerated integratedly. CONCLUSION: A. crenata sims can be regenerated integratedly and breeded fast by using axillary bud proliferation technology.
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