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  • Title: Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.
    Author: Hong J, Kang B, Kim A, Hwang S, Lee S, Kim J, Lee HY, Kang SH, Cheon DS.
    Journal: J Clin Microbiol; 2010 Feb; 48(2):615-6. PubMed ID: 19940056.
    Abstract:
    To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
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