These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Phosphate-functionalized magnetic microspheres for immobilization of Zr(4+) ions for selective enrichment of the phosphopeptides.
    Author: Qi D, Mao Y, Lu J, Deng C, Zhang X.
    Journal: J Chromatogr A; 2010 Apr 16; 1217(16):2606-17. PubMed ID: 19942223.
    Abstract:
    In this work, we developed phosphate functionalized magnetic Fe(3)O(4)@C microspheres to immobilize Zr(4+) ions for selective extraction and concentration of phosphopeptides for mass spectrometry analysis. Firstly, we synthesized Fe(3)O(4)@C magnetic microspheres as our previous work reported. Then, the microspheres were functionalized with phosphate groups through a simple hydrolysis reaction using 3-(trihydroxysilyl)propyl methylphosphate. And the Zr(4+) ions were immobilized on phosphate-functionalized magnetic microspheres by using phosphate chelator. Finally, we successfully employed Zr(4+)-phosphate functionalized magnetic microspheres to selectively isolate the phosphopeptides from tryptic digests of standard protein and real samples including rat brain. All the experimental results demonstrate the enrichment efficiency and selectivity of the method we reported here.
    [Abstract] [Full Text] [Related] [New Search]