These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Simultaneous HPLC determination of four triterpenoid acids in Ganoderma lucidum].
    Author: Zhao J, Chen X, Bi K.
    Journal: Zhongguo Zhong Yao Za Zhi; 2009 Sep; 34(17):2220-2. PubMed ID: 19943490.
    Abstract:
    OBJECTIVE: To determine 4 kinds of triterpenoid acids in Ganodermna lucidum, namely ganoderic acid C2, ganoderenic acid A, ganoderic acid A and ganoderic acid D quantitively. METHOD: The RP-HPLC method was applied and the separation was performed on a Kromasil C18 analytical column (4.6 mm x 250 mm, 5 microm). The mobile phase was acetonitrile (A)-water containing 0.03% H3PO4 (B) with gradient elution mode at the flow rate of 1.0 mL x min(-1). The detection was set at 252 nm, and the column temperature was 35 degrees C. RESULT: The linear ranges of ganoderic acid C2, ganoderenic acid A, ganoderic acid A and ganoderic acid D were 5.0-50.0 microg x mL(-1) (r = 0.9999), 7.2-72 mg x L(-1) (r = 0.9998), 11.67-116.7 mg x L(-1) (r = 0.9999), 5.32-53.2 mg x L(-1) (r = 0.9996), respectively. The average recoveries (n=9) were 98.8% (RSD 1.5%), 99.1% (RSD 1.9%), 99.5% (RSD 1.4%), 98.5% (RSD 1.9%), respectively. CONCLUSION: The method is simple and accurate with a good reproducibility and can be used as a quality control method for G. lucidum of different sources.
    [Abstract] [Full Text] [Related] [New Search]