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  • Title: Determination of phthalates in diet and bedding for experimental animals using gas chromatography-mass spectrometry.
    Author: Kondo F, Okumura M, Oka H, Nakazawa H, Izumi S, Makino T.
    Journal: Bull Environ Contam Toxicol; 2010 Feb; 84(2):212-6. PubMed ID: 19949769.
    Abstract:
    We have developed a gas chromatography-mass spectrometry method to measure five phthalates (dibutyl phthalate, butylbenzyl phthalate, di-2-ethylhexyl phthalate, diisooctyl phthalate, and diisononyl phthalate) in diets and beddings for experimental animals. The recoveries from diets and beddings spiked with five phthalates were 98.8%-148% with coefficients of variation of 0.4%-7.8% for diets and 94.7%-146% with coefficients of variation of 1.0%-5.0% for beddings. We analyzed commercial animal diets and beddings, and found that the levels of phthalates varied from sample to sample; the concentrations of five phthalates were 141-1,410 ng/g for diets and 20.5-7,560 ng/g for beddings.
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