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  • Title: Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica.
    Author: Wang S, Yu L, Che H, Chen X, Qin Y, Gao D, Liu M.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2009 Nov; 34(11):1063-9. PubMed ID: 19952394.
    Abstract:
    OBJECTIVE: To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum). METHODS: Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown. RESULTS: A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis. CONCLUSION: Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
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