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  • Title: Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VIII. Isolation and sequences of peptides from a tryptic hydrolysate of the maleylated protein.
    Author: Austen BM, Nyc JF, Brown DM, Smith EL.
    Journal: J Biol Chem; 1977 Nov 25; 252(22):8182-95. PubMed ID: 199603.
    Abstract:
    The NAD-specific glutamate dehydrogenase of Neurospora crassa was S-carboxymethylated with [14C]iodoacetate, maleylated, and hydrolyzed with trypsin. The isolation and sequences of the resulting peptides are described. These peptides gave information on the structure of the protein that was previously unknown and gave many overlaps of previously isolated segments of the protein.
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