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  • Title: Binding of LH and FSH to porcine granulosa cells during follicular maturation.
    Author: Nakano R, Akahori T, Katayama K, Tojo S.
    Journal: J Reprod Fertil; 1977 Sep; 51(1):23-7. PubMed ID: 199723.
    Abstract:
    The uptake of 125I-labelled LH by equal numbers of granulosa cells from small, medium or large follicles was greater by cells from large follicles. In contrast, granulosa cells obtained from small follicles bound much more 125I-labelled FSH per cell than did cells obtained from medium and large follicles. Competition studies with unlabelled hormones indicated that porcine granulosa cells have specific receptors for LH and FSH. The addition of diethylstilboestrol enhanced the binding of 125I-labelled LH and inhibited the binding of 125I-labelled FSH to granulosa cells harvested from small and medium-sized follicles, but had no effect on those from large follicles. The binding of iodine-125-labeled luteinizing hormone (LH) and follicle stimulating hormone (FSH) by procine granulosa cells at various stages of maturation was studied in vitro. Large follicles exhibited a greater uptake of labeled LH per cell than small and medium size follicles. However, small follicles bound more labeled FSH per cell than cells from medium and large size follicles. The presence of specific procine granulosa cell receptors for LH and FSH was established by competition studies with unlabeled hormones. The binding of labeled LH was enhanced by the addition of diethylstilbestrol, whereas the binding of labeled FSH to granulosa cells obtained from small and medium size, but not large, follicles was inhibited by diethylstilbestrol. It is suggested that estrogens may play an important role in the binding of LH and FSH to granulosa cells.
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