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  • Title: Rapid isolation of Mycobacteria--need of the hour in our settings.
    Author: Hannan A, Chaudhary S, Saleem S, Qayyum A, Arshad MU.
    Journal: J Ayub Med Coll Abbottabad; 2008; 20(4):67-9. PubMed ID: 19999208.
    Abstract:
    BACKGROUND: To compare Bactec MGIT 960 with LJ media in terms of time taken for the initial isolation of mycobacteria. METHODS: A total of 100 AFB (acid fast bacillus) positive sputum samples were processed and inoculated in both the Lowenstein Jensen (LJ) media and mycobacterium growth indicator tube (MGIT) tubes. RESULTS: Of the 100 samples, positive growth was obtained from all the samples on both the MGIT and LJ media. In MGIT 53% samples grew in 4 days, 30% in 5 days and 17% in 6 days (Mean = 4.6 days) while on LJ media, 44% grew in 30 days, 20% in 35 days and 36% in 44 days (Mean = 37 days). Significant difference was observed between two systems with a p-value of less than 0.05. CONCLUSION: Bactec MGIT 960 is a much faster and efficient system for the initial isolation of mycobacteria.
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