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  • Title: 25-OH-vitamin D assay variation and subject management in clinical practice.
    Author: Granado-Lorencio F, Mosteiro JS, Herrero-Barbudo C, Navarro ED, Blanco-Navarro I, Pérez-Sacristán B.
    Journal: Clin Biochem; 2010 Mar; 43(4-5):531-3. PubMed ID: 20004185.
    Abstract:
    OBJECTIVE: To compare two methods for assessing vitamin D status in clinical settings. DESIGN AND METHODS: 25-OH-vitamin D status was measured in 120 patients by HPLC and a commercial immunoassay. RESULTS: Only 53% of the subjects considered as vitamin D sufficient by immunoassay fell within this category according to HPLC although subjects with concentrations above 75 nmol/L, regardless of the method used, presented normal PTH concentrations. CONCLUSIONS: Both methods are not exchangeable to classify subjects based on unique cut-offs but they are comparable when interpreted in relation with PTH concentrations.
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